Journal of Agriculture and Food Science
Journal of Agriculture and Food Science. 2026; 6: (1) ; 10.12208/j.jafs.20260008 .
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1 集团研发中心，山东信得科技股份有限公司 山东青岛;
2 合肥市动物疫病预防控制中心 安徽合肥;
3 山东信得科技股份有限公司 山东诸城;
4 Department of Pathology, University of Agriculture Faisalabad, Pakistan;
5 莱阳市动物疫病预防控制中心 山东莱阳;
6 莱阳市高格庄畜牧兽医站 山东莱阳;
*通讯作者： 刘春国,单位： 集团研发中心，山东信得科技股份有限公司 山东青岛;；
病毒性疾病对养猪业造成了严重威胁，为了筛选有效消毒剂，优化猪场消毒方案，本研究采用荧光定量PCR方法，测定在不同稀释度、作用时间、环境温度及放置时间等条件下二氯异氰尿酸钠（DCCNa）对伪狂犬病毒（PRV）和猪流行性腹泻病毒（PEDV）的核酸降解效果。以Ct值≥36作为病毒核酸未检出的判定标准，提示病毒灭活。结果显示，在1：1000稀释条件下，DCCNa作用5 min即可使PRV与PEDV的Ct值达到≥36；在4 ℃、15 ℃及30 ℃条件下，DCCNa均能使两种病毒的Ct值≥36。然而，DCCNa溶液在常温敞口放置5 d后，有效氯含量下降至95%以下，病毒核酸降解能力随之减弱。DCCNa（三种剂型）与二氧化氯均表现出较佳的病毒核酸降解效果，优于过硫酸氢钾复合物粉剂、强力泡沫清洗溶剂和戊二醛癸甲溴铵溶剂。综上所述，DCCNa可作为猪场消毒体系建设的优选产品。本研究为猪场消毒剂的科学筛选与应用提供了可靠的数据支持，但需指出，本研究以qPCR Ct值作为间接指标，反映的是病毒核酸的降解或失活趋势，病毒是否完全丧失感染性尚需通过细胞培养实验进一步验证。
Viral diseases pose a severe threat to the swine industry. To screen for effective disinfectants and optimize disinfection protocols in pig farms, this study evaluated the nucleic acid degradation efficacy of sodium dichloroisocyanurate (DCCNa) against pseudorabies virus (PRV) and porcine epidemic diarrhea virus (PEDV) under various conditions, including different dilution ratios, reaction times, environmental temperatures, and storage times, using quantitative real-time PCR (qPCR). A cycle threshold (Ct) value ≥ 36 was set as the threshold for complete nucleic acid degradation, indicating potential virus inactivation. The results showed that DCCNa at a 1:1000 dilution achieved Ct values ≥ 36 for both PRV and PEDV within 5 min. DCCNa achieved complete nucleic acid degradation of both viruses at 4 °C, 15 °C, and 30 °C. However, when the DCCNa solution was left uncovered at room temperature for 5 days, the available chlorine content dropped below 95%, and its nucleic acid degradation efficacy subsequently decreased. Compared with four other commonly used disinfectants, DCCNa (in three formulations) and chlorine dioxide demonstrated superior nucleic acid degradation efficacy. DCCNa is recommended as a preferred product for establishing disinfection systems on pig farms. This study provides reliable data to support the scientific selection and application of disinfectants in swine farming. It should be noted that qPCR Ct values serve as an indirect indicator of viral nucleic acid degradation or inactivation; whether the virus has completely lost infectivity requires further validation through cell culture experiments.
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