International Research in Chinese Medicine
International Research in Chinese Medicine. 2026; 6: (1) ; 10.12208/j.ircm.20260004 .
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浙江中医药大学附属第二医院，浙江省新华医院 浙江杭州
*通讯作者： 陈梦静,单位：浙江中医药大学附属第二医院，浙江省新华医院 浙江杭州; ；
目的 观察归脾汤合甘麦大枣汤对CUMS模型小鼠抑郁样行为的改善作用，并基于“脑-肠轴”视角探讨其多靶点机制。方法 采用蔗糖偏好实验、强迫游泳实验及旷场实验评价行为学；ELISA检测血清5-HT、TNF-α、IL-10；16S rRNA测序分析肠道菌群；GC-MS测定粪便短链脂肪酸。结果 GMDZ-GP可显著升高蔗糖偏好率、缩短强迫游泳不动时间、延长中央区停留时间；上调血清5-HT水平，降低TNF-α水平，升高IL-10水平。菌群分析显示，GMDZ-GP恢复Shannon多样性，升高丁酸、丙酸含量。结论 GMDZ-GP通过“菌群-代谢-炎症-神经可塑性”网络整合调控脑-肠轴，发挥多靶点抗抑郁效应。
Objective To observe the effects of Gupi decoction and Ganmai Dazao decoction on depression-like behavior in CUMS model mice, and to explore the multi-target mechanism based on the perspective of “brain-gut axis”. Methods Sucrose preference test, forced swimming test and open field test were used to evaluate behavior. ELISA was used to detect serum 5-HT, TNF-α and IL-10. Gut microbiota was analyzed by 16S rRNA sequencing. Fecal short-chain fatty acids were determined by GC-MS. Results GMDZ-GP significantly increased sucrose preference rate, shortened the immobility time of forced swimming, and prolonged the time of staying in the central area. It upregulated the serum level of 5-HT, decreased the level of TNF-α and increased the level of IL-10. Microbiota analysis showed that GMDZ-GP restored Shannon diversity and increased the content of butyric acid and propionic acid. Conclusion GMDZ-GP integrates the brain-gut axis through the "microbiota-metabolism-inflammation-neuroplasticity" network to exert multi-target antidepressant effect.
[1] WANG C, PAN R, WAN X, et al. Immediate Psychological Responses and Associated Factors during the Initial Stage of the 2019 Coronavirus Disease (COVID-19) Epidemic among the General Population in China [J]. Int J Environ Res Public Health, 2020, 17(5): 1729.
[2] HUANG Y, WANG Y, WANG H, et al. Prevalence of mental disorders in China: a cross-sectional epidemiological study [J]. The lancet Psychiatry, 2019, 6(3): 211-24.
[3] （上古）黄帝等撰；李郁，任兴之编译；支旭仲主编. 黄帝内经 [M]. 西安：三秦出版社, 2018.
[4] 王永炎主编. 中医内科学 [M]. 上海：上海科学技术出版社, 1997.
[5] 程士德主编；王洪图，鲁兆麟编. 素问注释汇粹 [M]. 北京：人民卫生出版社, 1982.
[6] （宋）严用和撰. 济生方 [M]. 北京：人民卫生出版社, 1956.
[7] 庄红艳, 贾竑晓, 刘杰, et al. 甘麦大枣汤干预多种抑郁症的疗效及药理探究 [J]. 世界中医药, 2019, 14(07): 1907-10+14.
[8] 黄姗, 杨昆鹏, 万飞, et al. 甘麦大枣汤合归脾汤加减对PSD(心脾两虚证)的疗效及肠道菌群变化 [J]. 中国微生态学杂志, 2020, 32(10): 1173-6+81.
[9] 张晓美. 归脾汤治疗不寐合并焦虑抑郁临床观察 [J]. 中国中医药现代远程教育, 2021, 19(04): 94-6.
[10] 王晓歌, 周斌. 归脾汤联合中医情志护理对成人抑郁症患者血清5-羟色胺和去甲肾上腺素水平的影响 [J]. 河南中医, 2019, 39(08): 1213-6.