miRNA-140-5p inhibits the proliferation, migration and invasion of colorectal cancer SW480 cells by regulating FoxM1
miRNA-140-5p调控FoxM1抑制结直肠癌SW480细胞增殖、迁移及侵袭

摘要

目的 探讨miRNA-140-5p通过靶向调控FoxM1影响SW480细胞增殖、侵袭及迁移等生物学行为。方法 应用双荧光素酶报告基因系统鉴定对miR-140-5p和FoxM1的靶向匹配关系。CCK8检测FoxM1基因表达对细胞增殖的影响，以及miR-140-5p对FoxM1表达的调控作用。采用细胞集落形成实验检测FoxM1对细胞生长的影响，Western blotting检测FoxM1蛋白的表达，Transwell实验观察FoxM1对SW480细胞外的侵袭能力。结果 双荧光素酶报告基因系统鉴定表明miR-140-5p在转录后有效调控FoxM1的表达，CCK8检测表明FoxM1基因高表达可促进癌细胞的增殖，细胞集落形成实验检测结果表明FoxM1基因高表达能够增加SW480细胞的克隆能力，细胞划痕实验结果表明FoxM1基因在结直肠癌SW480细胞的迁移能力中起着重要作用，miR-140-5p抑制了细胞的迁移能力。结论 miR-140-5p可靶向调控FoxM1，敲低的FoxM1基因可抑制SW480细胞恶性化进程，miR-140-5p可能通过靶向调控FoxM1并参与SW480细胞增殖、侵袭及迁移等生物学行为。

Abstract

Objective To investigate the biological behaviors of SW480 cells such as proliferation, invasion and migration affected by miRNA-140-5p through targeted regulation of FoxM1.
Methods The dual luciferase reporter gene system was used to identify the targeted matching relationship between miR-140-5p and FoxM1. CCK8 was used to detect the effect of FoxM1 gene expression on cell proliferation, and the regulatory effect of miR-140-5p on FoxM1 expression. The effect of FoxM1 on cell growth was detected by cell colony formation assay, the expression of FoxM1 protein was detected by Western blotting, and the invasion ability of FoxM1 on SW480 cells was observed by Transwell assay.
Results Dual luciferase reporter gene system identification showed that miR-140-5p effectively regulated the expression of FoxM1 after transcription. CCK8 detection showed that high expression of FoxM1 gene could promote the proliferation of cancer cells. The results of cell colony formation assay showed that high expression of FoxM1 gene could increase the cloning ability of SW480 cells. The results of cell scratch assay showed that FoxM1 gene played an important role in the migration ability of colorectal cancer SW480 cells, and miR-140-5p inhibited the migration ability of cells.
Conclusion   miR-140-5p can target and regulate FoxM1. Knockdown of FoxM1 gene can inhibit the malignant process of SW480 cells. miR-140-5p may participate in the biological behaviors of SW480 cell proliferation, invasion and migration by targeting and regulating FoxM1.