International Journal of Clinical Research
International Journal of Clinical Research. 2024; 8: (10) ; 10.12208/j.ijcr.20240410 .
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黑龙江省中医药科学院针灸脑病五科 黑龙江哈尔滨
*通讯作者： 桑鹏,单位：黑龙江省中医药科学院针灸脑病五科 黑龙江哈尔滨；
目的 研究头穴透刺联合益智地黄汤（Reh）对脑梗死后痴呆（VD）治疗作用及对磷脂酰肌醇-3羟激酶(PI3K)/蛋白激酶B(AKT/PKB)调节氧化应激损伤和细胞凋亡的影响。方法 将小鼠分为4组：假手术组、VD组、多奈哌齐组、头穴透刺地黄汤组。采用水迷宫实验对学生的学习记忆能力进行评价。检测海马组织中caspase-3活性、MDA含量及SOD酶活性。体外将HT22细胞分为4组:假手术组、VD组、多奈哌齐组、头穴透刺地黄汤组。流式细胞术检测细胞内ROS含量及细胞凋亡。结果 与假手术组相比，VD组小鼠学习记忆能力明显下降。海马内p-AKT蛋白表达及SOD酶活性降低，Caspase-3活性及MDA含量明显升高。头穴透刺地黄汤组VD模型小鼠学习记忆能力明显提高，p-AKT蛋白表达及SOD酶活性上调， Caspase-3活性及MDA含量降低。结论 头穴透刺地黄汤通过调节PI3K/AKT途径来减轻氧化应激，抑制细胞凋亡以改善脑功能。
Objective To study the therapeutic effect of scalp acupuncture combined with Yizhi Reh decoction (Reh) on post-stroke dementia (VD) and its effect on phosphatidylinositol-3-hydroxykinase (PI3K)/protein kinase B (AKT/PKB) regulating oxidative stress injury and cell apoptosis. Methods Mice were divided into 4 groups: sham operation group, VD group, donepezil group, and scalp acupuncture Reh decoction group. The water maze test was used to evaluate the learning and memory ability of students. The activity of caspase-3, the content of MDA and the activity of SOD in hippocampal tissue were detected. HT22 cells were divided into 4 groups in vitro: sham operation group, VD group, donepezil group, and scalp acupuncture Reh decoction group. Flow cytometry was used to detect the content of intracellular ROS and cell apoptosis. Results Compared with the sham operation group, the learning and memory ability of mice in the VD group was significantly decreased. The expression of p-AKT protein and SOD enzyme activity in the hippocampus decreased, while the activity of Caspase-3 and the content of MDA increased significantly. The learning and memory ability of VD model mice in the head-point penetrating Rehmannia decoction group was significantly improved, the expression of p-AKT protein and SOD enzyme activity were upregulated, and the activity of Caspase-3 and the content of MDA decreased. Conclusion Head-point penetrating Rehmannia decoction can reduce oxidative stress and inhibit cell apoptosis to improve brain function by regulating the PI3K/AKT pathway.
[1] N. Munoz-Rivas, M. Mendez-Bailon, J.M. Miguel-Yanes, V. Hernandez-Barrera, J. Miguel-Diez, R. Jimenez-Garcia, and A. Lopez-de-Andres, Observational study of vascular dementia in the Spanish elderly population according to type 2 diabetes status: trends in incidence, characteristics and outcomes (2004-2013), BMJ Open 7, e016390 (2017).
[2] A.R. Borenstein, Y. Wu, J.D. Bowen, W.C. McCormick, J. Uomoto, S.M. McCurry, G.D. Schellenberg, and E.B. Larson, Incidence rates of dementia, Alzheimer disease, and vascular dementia in the Japanese American population in Seattle, WA: the Kame Project, Alzheimer Dis Assoc Disord 28, 23-29 (2014).
[3] Z. Lin, J. Gu, J. Xiu, T. Mi, J. Dong, and J.K. Tiwari, Traditional chinese medicine for senile dementia, Evid Based Complement Alternat Med 2012, 692621 (2012).
[4] H. Yan, L. Li, and X.C. Tang, Treating senile dementia with traditional Chinese medicine, Clin Interv Aging 2, 201-208 (2007).
[5] E.S. Chan, D.T. Bautista, Y. Zhu, Y. You, J.T. Long, W. Li, and C. Chen, Traditional Chinese herbal medicine for vascular dementia, Cochrane Database Syst Rev 12, CD010284 (2018).
[6] X.J. Li, and H.Y. Zhang, Potential anti-dementia agents in traditional Chinese medicine, Nat Prod Commun 4, 877-886 (2009).
[7] 毕天威, 徐子绚, 宋杰,等. 基于TLR-4/MyD88/NF-κB通路探讨针刺改善血管性痴呆大鼠认知功能的机制[J]. 世界科学技术-中医药现代化,2023,25(12):4032-4038.
[8] 刘传珍, 周丽华, 水正. 通窍活血汤合补阳还五汤治疗血管性痴呆36例临床研究[J]. 中医杂志,2002,43(7):526-527.
[9] 师会, 赵锐. 补肾活血法治疗血管性痴呆的理论探讨[J]. 辽宁中医杂志,2015,42(2):266-268.
[10] 郝静, 白龙跃, 牛志学,等. 益气补肾活血化痰方联合常规治疗对肾虚血瘀、痰浊阻滞型血管性痴呆认知功能障碍患者的临床疗效[J]. 中成药,2022,44(03):789-793.
[11] 艾艳萍, 丁涛, 蔡桃英. 天麻素对急性缺血性脑卒中后痴呆患者的疗效分析[J]. 西南国防医药,2018,28(11):1048-1051.
[12] 白春艳, 赵丽宏. 长春西汀注射液治疗脑卒中后痴呆的临床疗效分析[J]. 中国医药指南,2013(29):129-130.