Effects of Wendan Decoction on sleep conditions and neurotransmitter levels in the hippocampus of insomniac rats
温胆汤对失眠大鼠睡眠情况及海马区神经递质水平的影响

摘要

目的 探讨温胆汤对对氯苯丙氨酸（PCPA）所导致的失眠大鼠睡眠情况以及海马区域神经递质水平的影响。方法 将40只SD大鼠随机抽取10只设为正常组，另30只予腹腔注射PCPA混悬液制备失眠模型，造模成功后随机分为模型对照组、艾司唑仑组、温胆汤组，每组10只。各给药组连续7D予相应浓度药物灌胃，每天1次，正常组和模型对照组连续7D予等量生理盐水灌胃。在给药第7D，灌胃30min后行戊巴比妥钠催眠大鼠，观察各组睡眠潜伏期和睡眠时间。诱导睡眠结束后将各组大鼠禁食不禁水12小时，然后通过腹腔注射戊巴比妥钠进行麻醉，分离其海马组织进行5-HT、GLU、GABA指标的检测。结果 温胆汤组大鼠的一般状态优于模型对照组，且体质量变化较模型对照组上升（P<0.05）；与模型对照组相比，温胆汤组大鼠的睡眠潜伏期明显缩短（P<0.05），睡眠时间明显延长(P<0.05),温胆汤组海马区5-羟色胺（5-HT）、γ-氨基丁酸（GABA）的含量明显下降（P<0.05），谷氨酸（Glu）的水平显著升高（P<0.05）。与模型对照组比较，各药物组大鼠的失眠症状有所改善，上述指标虽不及正常组，但皆有趋向正常组的趋势（P<0.05）。结论 温胆汤具有改善PCPA所致的失眠大鼠睡眠障碍的作用。

Abstract

Objective To investigate the effects of Wendan Decoction on sleep and neurotransmitter levels in the hippocampal region of p-chlorophenylalanine (PCPA)-induced insomnia rats.
Methods Ten of 40 SD rats were randomly selected as the normal group, and the other 30 rats were injected intraperitoneally with PCPA suspension to prepare the insomnia model, and then randomly divided into the model control group, the eszopiclone group, and the Wendan Decoction group, with 10 rats in each group. Each group was given the corresponding concentration of the drug by gavage for 7 consecutive days, and the normal group and the model control group were given the same amount of saline by gavage for 7 consecutive days. On the 7th day of drug administration, the rats were hypnotised with sodium pentobarbital after 30 min of gavage, and the sleep latency and sleep time of each group were observed. After sleep induction, the rats in each group were fasted for 12 hours, anaesthetized by intraperitoneal injection of sodium pentobarbital, and their hippocampal tissues were separated for the detection of 5-HT, GLU, and GABA indexes.
Results The general state of the rats in the Wendan Decoction group was better than that of the model control group, and the change of body mass increased compared with that of the model control group (P<0.05); compared with the model control group, the sleep latency of the rats in the Wendan Decoction group was significantly shortened (P<0.05), and the sleeping time was significantly prolonged (P<0.05), and the levels of 5-hydroxytryptamine (5-HT) and γ-aminobutyric acid (GABA) in the hippocampus of the Wendan Decoction group decreased (P<0.05), and the level of glutamic acid (Glu) was significantly increased (P<0.05). decreased (P<0.05), and the level of glutamate (Glu) was significantly increased (P<0.05). Compared with the model control group, the insomnia symptoms of rats in each drug group were improved, and the above indexes were not as good as those of the normal group, but all of them had a tendency to converge to the normal group (P<0.05).
Conclusion   Wendan Decoction has the effect of improving sleep disorder in PCPA-induced insomnia rats.