International Research in Chinese Medicine
International Research in Chinese Medicine. 2024; 4: (4) ; 10.12208/j.ircm.20240054 .
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1 南京中医药大学附属苏州市中医医院 江苏苏州
2 苏州市吴门医派验方评价与转化重点实验室 江苏苏州
*通讯作者： 蒋春波,单位： 南京中医药大学附属苏州市中医医院 江苏苏州；
目的 基于miRNA-30b-3p介导Wnt信号通路研究吴门肾炎口服液 Ⅰ 对阿霉素诱导大鼠足细胞损伤的保护作用。方法 建立阿霉素肾足细胞损伤的大鼠模型，采用生化法、电镜学和蛋白质免疫印迹等研究方法，结合miRNA模拟物转染技术，通过分组干预，探讨吴门肾炎口服液 Ⅰ 干预miRNA-30b-3p调控Wnt/β-catenin信号通路进而保护足细胞的作用。结果 与模型组相比，吴门肾炎口服液 Ⅰ 显著降低24 h蛋白尿（P < 0.01）；病理学结果显示其改善阿霉素诱导大鼠足细胞损伤，减轻肾脏病理损伤，与其抑制阿霉素诱导的肾足细胞Nephrin和Podocin的蛋白表达降低（P < 0.01）相关；另外，与模型组相比，miR-30b-3p过表达质粒注射组及肾炎口服液 Ⅰ + miR-30b-3p过表达质粒注射组的大鼠肾脏中Wnt1、β-catenin的表达水平明显下调（P < 0.05或P < 0.01）。结论 吴门肾炎口服液 Ⅰ 可能通过上调miR-30b-3p表达，抑制Wnt/β-catenin信号通路活化，从而改善阿霉素诱导的大鼠足细胞功能障碍。
Objective The protective effect of Wumen Shenyan Oral Liquid Ⅰ on adriamycin-induced podocyte injury in rats was studied based on the miRNA-30b-3p-mediated Wnt signaling pathway. Methods The rat model of adriamycin-induced renal podocyte injury was established. Biochemical method, electron microscopy and Western blotting were used to investigate the effect of Wumen Shenyan Oral Liquid I on miRNA-30b-3p regulating Wnt/β-catenin signaling pathway and protecting podocytes by group intervention combined with miRNA mimic transfection technology. Results Compared with the model group, Wumen Nephritis Oral Liquid I significantly reduced 24 h proteinuria (P< 0.01), improved podocyte injury, and reduced renal pathological damage. The protein expression of Nephrin and Podocin in renal podocytes induced by adriamycin was inhibited (P < 0.01). In addition, compared with the model group, the expression levels of Wnt1 and β-catenin in the kidneys of rats in the miR-30b-3p overexpression plasmid injection group and the nephritis oral liquid I + miR-30b-3p overexpression plasmid injection group were significantly down-regulated (P< 0.05 or P< 0.01). Conclusion Wumen Shenyan Oral Liquid I may improve adriamycin-induced podocyte dysfunction in rats by up-regulating the expression of miR-30b-3p and inhibiting Wnt /β-catenin signaling pathway.
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