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国际临床研究杂志

International Journal of Clinical Research

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International Journal of Clinical Research. 2023; 7: (11) ; 10.12208/j.ijcr.20230342 .

Detection of lymphocyte subsets in peripheral blood of patients with colonic polyps and its clinical significance
结肠息肉患者外周血淋巴细胞亚群检测及临床意义

作者： 张秋心, 郑盛 *, 杨涓

大理大学第二附属医院消化内科 云南昆明

*通讯作者： 郑盛,单位：大理大学第二附属医院消化内科 云南昆明；

引用本文: 张秋心, 郑盛, 杨涓 结肠息肉患者外周血淋巴细胞亚群检测及临床意义[J]. 国际临床研究杂志, 2023; 7: (11) : 10-15.
Published: 2023/11/21 17:45:32

摘要

目的 探讨结肠息肉患者外周血淋巴细胞亚群的变化及临床意义。方法 回顾性分析2022年3月-2023年4月就诊于云南省第三人民院消化内科的130例病人标本，分为结肠息肉组（75例）和健康对照组（55例），采用流式细胞技术检测其外周血淋巴细胞亚群，比较两组间T、B、NK 淋巴细胞亚群绝对计数与百分比的差异性；将结肠息肉组分为非腺瘤性息肉（34例）和腺瘤性息肉（41例），比较两组间T、B、NK 淋巴细胞亚群绝对计数与百分比的差异性。结果 结肠息肉组CD19+B淋巴细胞百分比[12.60（9.30，16.80）]%、CD3+CD4+T淋巴细胞绝对数目[582（435，811）]个/（ul）、CD45+LYM淋巴细胞绝对计数[1484（1134，1788）]个/（ul）、CD3+总T淋巴细胞绝对数目[964（731，1217）]个/（ul）、CD19+ B淋巴细胞绝对数目[168（118，270）]个/（ul）均低于健康对照组[13.5（11.5，19.2）]%，[670（534，798）]个/（ul），[1556（1353，2023）]个/（ul），[221（173，329）]个/（ul），差异具有统计学意义（t=-2.40，-2.13，-2.26，-2.01，-3.23，均P＜0.05）；其余结果差异均无统计学意义（均 P >0.05）。结论 结肠息肉患者CD19+B淋巴细胞百分比、CD19+#B淋巴细胞绝对数目、CD3+CD4+#辅助/诱导T淋巴细胞绝对数目、CD45+LYM#淋巴细胞绝对计数、CD3+#总T淋巴细胞绝对数目均有所降低，提示结肠息肉发生发展与机体免疫力相关，淋巴细胞亚群部分指标对结肠息肉筛查具有一定临床意义。

关键词： 结肠息肉；结肠癌；T淋巴细胞；B淋巴细胞；自然杀伤细胞

Abstract

Objective To investigate the changes and clinical significance of peripheral blood lymphocyte subsets in patients with colonic polyps.
Methods From March 2022 to April 2023, 130 cases of patients in the Department of Gastroenterology of the Third People's Hospital of Yunnan Province were retrospectively analyzed, which were divided into colon polyp group (75 cases) and healthy control group (55 cases). The lymphocyte subsets in peripheral blood were detected by flow cytometry, and the differences of absolute counts and percentages of T, B and NK lymphocyte subsets between the two groups were compared. Colon polyp group was divided into non-adenomatous polyp group (34 cases) and adenomatous polyp group (41 cases), and the difference of absolute count and percentage of T, B and NK lymphocyte subsets between the two groups was compared .
Results In colon polyp group, the percentage of CD19+B lymphocytes [12.60(9.30,16.80)]%, the absolute number of CD3+CD4+T lymphocytes [582(435,811)] /(ul) and the absolute count of CD45+LYM lymphocytes [1484 (1134,1788),The absolute numbers of CD3+ total T lymphocytes [964(731,1217)] /(ul) and CD19+ B lymphocytes [168(118,270)] /(ul) were lower than those of healthy controls [13.5(11.5,19.2)]%, [670(534,798)]/（ul）,[1556(1353,2023)]/(ul),[221(173,329)]/(ul),the difference was statistically significant (t=-2.40, -2.13, -2.26, -2.01, -3.23, all P < 0.05)；There was no significant difference in other results (all P >0.05).
Conclusion   The percentage of CD19+B lymphocytes, the absolute number of CD19+#B lymphocytes, the absolute number of CD3+CD4+# helper/inducer T lymphocytes, the absolute number of CD45+LYM# lymphocytes and the absolute number of CD3+# total T lymphocytes in patients with colonic polyps all decreased, suggesting that the occurrence and development of colonic polyps are related to immunity. Some indicators of lymphocyte subsets have certain clinical significance for screening colonic polyps.

Key words： Colonic polyp; Colon cancer; T lymphocytes; B lymphocytes; Natural killer

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