Journal of Cell and Molecular Biology Research
Journal of Cell and Molecular Biology Research. 2022; 2: (1) ; 10.12208/j.ijcmbr.20220006 .
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南通大学 江苏南通
*通讯作者： 张华,单位：南通大学 江苏南通；
基因组编辑中CRISPR/Cas系统应用比较广泛，CRISPR/Cas系统能准确切割、识别DNA、RNA序列，在对目标序列识别过程中，CRISPR/Cas系统在Cas12、13蛋白经RNA引导，不仅能对目标序列进行识别，让靶向切割活性得到启动，且在DNA、RNA分子及其他单链中也表现出非特异性切割活性，将荧光基团报告核酸分子加入反应体系，通过荧光信号还能观察到目标核酸分子所在，且借助Cas蛋白的不同切割偏好，还能同时检测多种靶标分子。
The CRISPR/Cas system is widely used in genome editing. The CRISPR/Cas system can precisely cleave and recognize DNA and RNA sequences. In the process of recognizing a target sequence, the CRISPR/Cas system not only recognizes the target sequence and initiates target-cleaving activity, but also shows non-specific cleavage activity on single strands such as DNA and RNA molecules, and emits fluorescence. is appended. Group of reporter nucleic acid molecules to be added In the reaction system, the positions of target nucleic acid molecules can be observed by fluorescence signals, and multiple target molecules can be detected simultaneously due to the different cleavage priorities of Cas proteins.
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