Analysis of the diagnostic effect of PCR detection of 488 cases of rubella virus from 2007 to 2022
2007-2022年PCR检测488例风疹病毒的诊断效果分析

摘要

目的 探讨PCR检测风疹病毒的效果，判断其应用价值。方法 按照研究纳入标注与排除标准从2007年1月-2022年6月内本院收治的风疹患者中选取488例作为研究对象，所有患者病历资料完整，编号1-488号，所有的患者均同时接受PCR检测以及ELISA检测，PCR检测结果视为研究组数据，ELISA检测数据视为对照组数据，对比两组数据的检查符合率、特异度以及敏感度。结果 研究以细胞培养法为金标准，488例患者呈风疹病毒阳性，在临床结合病例资料分析中，PCR检查符合率高于ELISA抗体检测，同时PCR检查方法的敏感度显著更高，差异有统计学意义（P<0.05），两种检查方法的特意度，由于真阴性0例，故不涉及计算。结论 应用荧光定量PCR检查操作便捷，检查准确率高、敏感性与特异性高，定量准确，能够有效检出风疹病毒感染，为临床治疗和传染预防提供有效的参考信息，PCR检测临床效果好，有推广应用价值。

Abstract

Objective To explore the effect of PCR detection of rubella virus and judge its application value.
Methods according to the inclusion and exclusion criteria of the study, 488 rubella patients admitted to our hospital from January 2007 to June 2022 were selected as the study subjects. All patients had complete medical records, numbered 1-488. All patients underwent PCR and ELISA tests at the same time. The PCR test results were regarded as the data of the study group, and the ELISA test data were regarded as the data of the control group. The coincidence rate, specificity and sensitivity of the two groups of data were compared.
Results the cell culture method was used as the gold standard in the study. 488 patients were rubella virus positive. In the clinical analysis combined with case data, the coincidence rate of PCR examination was higher than that of ELISA antibody detection. At the same time, the sensitivity of PCR examination method was significantly higher, and the difference was statistically significant (P < 0.05). Since there were 0 true negative cases, the two examination methods did not involve calculation.
Conclusion   the application of fluorescence quantitative PCR is convenient, with high accuracy, sensitivity and specificity, and accurate quantification. It can effectively detect rubella virus infection and provide effective reference information for clinical treatment and infection prevention. The clinical effect of PCR detection is good, and it has the value of popularization and application.